ti-eclipse epifluorescent microscope Search Results


ti eclipse epifluorescence inverted microscope  (Nikon)
99
Nikon ti eclipse epifluorescence inverted microscope
(A) Visualization of pUL37-EGFP during HSV1 infection of human foreskin fibroblasts using live cell <t>epifluorescence</t> microscopy. Images show a representative field of infected cells at 8 and 20 hpi. Zoomed images show localization of pUL37-EGFP (green) in the same cell at 8 and 20 hpi. Scale bar = 50 µm. (B) IP-MS workflow. Human fibroblasts were synchronously infected (multiplicity of infection = 10) with either pUL37-EGFP or EGFP HSV1, with two replicates per condition. HSV1-UL37GFP was collected at 8 and 20 hpi and HSV1-GFP at 20 hpi (HSV1-GFP). pUL37-EGFP and its interactions were isolated from the cytoplasmic cell fraction by immunoaffinity purification using anti-GFP antibodies. Proteins were digested with trypsin, and the resulting peptides from each sample were labelled with unique TMT reagents and then combined prior to nanoliquid chromatography-tandem mass spectrometry analysis. (C) The recovery of pUL37-EGFP and EGFP in the immunoisolates was assessed by western blot with an anti-GFP antibody. 10% of each sample was analysed. (D) PPIs around pUL37 present in the reconstructed HSV1 network and supported by immunoaffinity purification results. (E) The abundance of pUS10 interaction with pUL37 at 8 and 20 hpi (average ± range, N=2). The relative amount of pUS10 was calculated by TMT-MS quantification and normalized by the respective pUL37 TMT abundance in each IP.
Ti Eclipse Epifluorescence Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ti eclipse epifluorescence inverted microscope/product/Nikon
Average 99 stars, based on 1 article reviews
ti eclipse epifluorescence inverted microscope - by Bioz Stars, 2026-03
99/100 stars
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ti eclipse epifluorescence microscope  (Nikon)
96
Nikon ti eclipse epifluorescence microscope
(A) Visualization of pUL37-EGFP during HSV1 infection of human foreskin fibroblasts using live cell <t>epifluorescence</t> microscopy. Images show a representative field of infected cells at 8 and 20 hpi. Zoomed images show localization of pUL37-EGFP (green) in the same cell at 8 and 20 hpi. Scale bar = 50 µm. (B) IP-MS workflow. Human fibroblasts were synchronously infected (multiplicity of infection = 10) with either pUL37-EGFP or EGFP HSV1, with two replicates per condition. HSV1-UL37GFP was collected at 8 and 20 hpi and HSV1-GFP at 20 hpi (HSV1-GFP). pUL37-EGFP and its interactions were isolated from the cytoplasmic cell fraction by immunoaffinity purification using anti-GFP antibodies. Proteins were digested with trypsin, and the resulting peptides from each sample were labelled with unique TMT reagents and then combined prior to nanoliquid chromatography-tandem mass spectrometry analysis. (C) The recovery of pUL37-EGFP and EGFP in the immunoisolates was assessed by western blot with an anti-GFP antibody. 10% of each sample was analysed. (D) PPIs around pUL37 present in the reconstructed HSV1 network and supported by immunoaffinity purification results. (E) The abundance of pUS10 interaction with pUL37 at 8 and 20 hpi (average ± range, N=2). The relative amount of pUS10 was calculated by TMT-MS quantification and normalized by the respective pUL37 TMT abundance in each IP.
Ti Eclipse Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ti eclipse epifluorescence microscope/product/Nikon
Average 96 stars, based on 1 article reviews
ti eclipse epifluorescence microscope - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier

Image Search Results


(A) Visualization of pUL37-EGFP during HSV1 infection of human foreskin fibroblasts using live cell epifluorescence microscopy. Images show a representative field of infected cells at 8 and 20 hpi. Zoomed images show localization of pUL37-EGFP (green) in the same cell at 8 and 20 hpi. Scale bar = 50 µm. (B) IP-MS workflow. Human fibroblasts were synchronously infected (multiplicity of infection = 10) with either pUL37-EGFP or EGFP HSV1, with two replicates per condition. HSV1-UL37GFP was collected at 8 and 20 hpi and HSV1-GFP at 20 hpi (HSV1-GFP). pUL37-EGFP and its interactions were isolated from the cytoplasmic cell fraction by immunoaffinity purification using anti-GFP antibodies. Proteins were digested with trypsin, and the resulting peptides from each sample were labelled with unique TMT reagents and then combined prior to nanoliquid chromatography-tandem mass spectrometry analysis. (C) The recovery of pUL37-EGFP and EGFP in the immunoisolates was assessed by western blot with an anti-GFP antibody. 10% of each sample was analysed. (D) PPIs around pUL37 present in the reconstructed HSV1 network and supported by immunoaffinity purification results. (E) The abundance of pUS10 interaction with pUL37 at 8 and 20 hpi (average ± range, N=2). The relative amount of pUS10 was calculated by TMT-MS quantification and normalized by the respective pUL37 TMT abundance in each IP.

Journal: bioRxiv

Article Title: Consensus clustering of herpesvirus protein interaction networks provides insights into their evolutionary relationship with bacteriophages

doi: 10.1101/473751

Figure Lengend Snippet: (A) Visualization of pUL37-EGFP during HSV1 infection of human foreskin fibroblasts using live cell epifluorescence microscopy. Images show a representative field of infected cells at 8 and 20 hpi. Zoomed images show localization of pUL37-EGFP (green) in the same cell at 8 and 20 hpi. Scale bar = 50 µm. (B) IP-MS workflow. Human fibroblasts were synchronously infected (multiplicity of infection = 10) with either pUL37-EGFP or EGFP HSV1, with two replicates per condition. HSV1-UL37GFP was collected at 8 and 20 hpi and HSV1-GFP at 20 hpi (HSV1-GFP). pUL37-EGFP and its interactions were isolated from the cytoplasmic cell fraction by immunoaffinity purification using anti-GFP antibodies. Proteins were digested with trypsin, and the resulting peptides from each sample were labelled with unique TMT reagents and then combined prior to nanoliquid chromatography-tandem mass spectrometry analysis. (C) The recovery of pUL37-EGFP and EGFP in the immunoisolates was assessed by western blot with an anti-GFP antibody. 10% of each sample was analysed. (D) PPIs around pUL37 present in the reconstructed HSV1 network and supported by immunoaffinity purification results. (E) The abundance of pUS10 interaction with pUL37 at 8 and 20 hpi (average ± range, N=2). The relative amount of pUS10 was calculated by TMT-MS quantification and normalized by the respective pUL37 TMT abundance in each IP.

Article Snippet: The progression of infection was visualized by live-cell imaging on a Nikon Ti-Eclipse epifluorescence inverted microscope from 2 hpi to 24 hpi.

Techniques: Infection, Epifluorescence Microscopy, Isolation, Immunoaffinity Purification, Chromatography, Mass Spectrometry, Western Blot